Charles S. Boyer

Name: Diatomaceae of Philadelphia — Appendix
Author: Charles S. Boyer

Diatomaceae of Philadelphia — Appendix (1916)

Summary

“Diatomaceae of Philadelphia — Appendix”, published in 1916, is a work by Charles S. Boyer. It explores elements such as diatoms, slide and bottle.

Quotes from Diatomaceae of Philadelphia — Appendix (Charles S. Boyer)

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ With a small forceps it is possible to detach minute quantities of a pure gathering which may not need further preparation beyond burning to a red heat on the cover-glass before mounting. A malacca cane, with extending rod to which may be screwed a bottle, net, spoon or hook, is useful on a long trip. If it is impossible to separate the thin film of diatoms from the mud in the bed of streams, dip up the surface mud with one bottle, allow to settle a few minutes, then pour off the supernatant liquid, which will be comparatively free from sand, into another bottle. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ In the case of partly siliceous species, washing in pure water repeatedly is all that can be done. The larger and heavier diatoms may be separated from the sand by elutriation or by whirling in a casserole, by rocking in a shallow dish the shape of a watch crystal, or by pouring slowly over a strip of plate-glass at least two feet in length inclined at an angle of thirty degrees. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ Now carefully lower the body-tube of the instrument until the diatom rests upon the gelatine, breathe gently upon it, remove the cover from the slide, invert it over another slide containing a drop of styrax and proceed by heating to mount as before. The size of the diatom, the amount of gelatine, and several other factors, will enter into the question of success or failure. I have, however, employed the above method and have mounted thousands of slides of selected diatoms successfully. It is necessary to avoid any air current which will cause the diatom to fall from the thread. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ When satisfied with the cleaning, preserve the stock material in part alcohol and, in using, pour into a smaller bottle the amount required, replace the dilute alcohol with distilled water, and mount as directed. It often happens that gatherings are made consisting almost entirely of sand. Attempts at cleaning in the usual way will cause the loss of nearly all of the diatoms. In this case, after the material has been treated with acid until nothing remains but sand and a few diatoms, the mechanical finger must be used. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ By repeating the decantation, shaking and whirling, the deposit will be found to consist almost entirely of diatoms. It may be necessary to repeat the boiling in the acid and bichromate. If, however, any detritus other than sand is noted, boil in sulphuric acid and add from time to time minute pinches of powdered chlorate of potash, being careful to protect the eyes by holding a piece of glass before them; otherwise the explosions which occur are likely to throw some of the boiling acid into the eyes and destroy the sight. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ By the same method, slides of arranged diatoms can be made using a glass circle properly marked with lines in the eye-piece. Care should be taken to use glass threads more or less in proportion to the size of the diatoms. A cat's whisker is preferred by some to the glass thread. It has the advantage of not breaking, but unless it is quite short it is too flexible. If the point of the thread becomes covered with gelatine, lower it into a minute drop of water upon a separate slide, and by moving it about it will be cleaned. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ Collection of Marine Material.—Shell scrapings, the stomachs of fish, marine algæ, especially the brown and red algæ, the hulls of vessels, mud from anchors and dredgings, are all sources which may prove valuable. ”

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Source: Wikisource

Charles S. Boyer, Diatomaceae of Philadelphia — Appendix (1916)

“ For selecting with the mechanical finger, an objective of two-thirds-inch focus is the most convenient, but for determining species a one-fifth-inch is needed, an immersion objective being essential for minute forms. ”

Charles S. Boyer

Summary

Quotes from Diatomaceae of Philadelphia — Appendix (Charles S. Boyer)

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