Cheikh Tidiane Diagne; Diawo Diallo; Oumar Faye; Yamar Ba; Ousmane Faye; Alioune Gaye; Ibrahima Dia; Ousmane Faye; Scott C. Weaver; Amadou Alpha Sall; Mawlouth Diallo

“ For each population, several breeding habitats were prospected and collected eggs reared in the laboratory. Females F0 were fed several times on guinea pigs to obtain F1 generation eggs. These eggs were hatched and the larvae reared to F1 adults used in this study. ”

“ Infection, dissemination and transmission rates at different incubation period for different mosquito species orally exposed with six zika virus strains The overall trend has been the low dissemination in the populations of Ae. ”

“ After trituration, pools were centrifuged at 7500 rpm for 10 min at 4 °C. For each sample, 100 μl of supernatant were used for RNA extraction with the QiaAmp Viral RNA Extraction Kit (Qiagen, Heiden, Germany) according to the manufacturer’s protocol with slight modification [20] . ”

“ The authors gratefully thank Amadou Thiaw, Abdou K. Bodian for their technical assistance during fieldwork and mosquito rearing.
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“ Culicidae) for Usutu virus. Am J Trop Med Hyg. 2012;86 (6) :993–996.10.4269/ajtmh.2012.11-050922665607↑ Fall G, Diallo M, Loucoubar C, Faye O. Vector competence of Culex neavei and Culex quinquefasciatus (Diptera: Culicidae) from Senegal for lineages 1, 2, Koutango and a putative new lineage of West Nile virus. Am J Trop Med Hyg. 2014;90 (4) :747–754.10.4269/ajtmh.13-040524567319↑ Chen R, Wang E, Tsetsarkin KA, Weaver SC. Chikungunya virus 3’ untranslated region: adaptation to mosquitoes and a population bottleneck as major evolutionary forces. ”