Oumar Faye; Ousmane Faye; Diawo Diallo; Mawlouth Diallo; Manfred Weidmann; Amadou Alpha Sall

“ Then, serological and entomological Zika infection were reported in different area in Africa (Burkina Faso, Ivory Cost, Egypt, Central African Republic, Mozambic, Nigeria, Uganda, Central African Republic and Senegal) [2] , [11] , [12] - [13] . Between 1968 and 2002, 606 strains including 10 human ZIKV strains were isolated in Central and West Africa countries and reported by the WHO Collaborating Center for Arbovirus and Viral Hemorrhagic Fever of Pasteur Institute in Dakar [14] . ”

“ Alignment of the designed primers and probe with Zika virus strain sequences. Dots indicate identity with the consensus sequence on the top of the alignment. Sensitivity and specificity of the rRT-PCR assay
The assay detected the RNA of all 37 ZIKV strains (Table 1) . The detection limit of the assay was evaluated using a quantitative RNA standard and a pfu-dilution series. To standardize the assay, serial dilutions of transcribed ZIKV RNA were tested by the real time PCR developed (Table 4) . ”

“ The NS5 protein is constituted by two distinct domains, an N-terminal methyltransferase and a C-terminal RNA-dependent RNA polymerase that are required for capping and synthesis of the viral RNA genome, respectively [5] - [6] . ”

“ Emerg Infect Dis. 2008;10 (8) :1232–1239.10.3201/eid1408.080287186806461 2 3 Foy BD, Kobylinski KC, Foy JLC, Blitvich BJ, Travassos da Rosa A, Haddow AD, Lanciotti RS, Tesh RB. Probable non–vector-borne transmission of Zika virus, Colorado USA. Emerg Infect Dis. 2011;10:5.1 2 3 Digoutte JP, Calvo-Wilson MA, Mondo M, Traoré-Lamizana M, Adam F. Continuous cell lines immune ascite fluid pools in arbovirus detection. ”

“ Am J Trop Med Hyg. 2008;10 (4) :505–510.18840736↑ Haddow A, Schuh AJ, Yasuda CY, Kasper MR, Heang V, Huy R, Guzman H, Tesh RB, Weaver SC. Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Negl Trop Dis. 2012;10 (2) :1–7.↑ Leland DS, Ginocchio CC. Role of cell culture for virus detection in the age of technology. Clin Microbiol Rev. 2007;10 (1) :49–78.10.1128/CMR.00002-0617223623↑ Simpson DL. Zika virus infection in man. ”