National Institute for Occupational Safety and Health

“ Inject 100 μL sample extract with a high-pressure syringe or a sampling loop. Record the volume injected to the nearest 0.5 μL. Under these conditions, the retention times are 3.6 min for Bromoxynil and 33 min for Bromoxynil octanoate. Measure peak area. NOTE: If the peak area exceeds the linear range of the system, dilute with acetonitrile and reanalyze. If the peak area measurement is hindered by the presence of interferences, other chromatographic conditions may be required. ”

“ Bromoxynil. Bromoxynil octanoate. Acetonitrile, HPLC grade. Water, distilled, deionized. Calibration stock solution, 1 mg/mL. Dissolve 0.100 g each of Bromoxynil and Bromoxynil octanoate in acetonitrile; dilute to 100 mL in a volumetric flask. Prepare daily, in duplicate.
1. Sampler: PTFE-coated glass fiber (Pallflex T60A20) or 2-μm PTFE membrane (GHIA) , 37-mm diameter. (Filters commercially available) . NOTE: Use opaque or light-protected cassette filter holder. 2. Personal sampling pump, 1 to 3 L/min, with flexible connecting tubing. 3. ”

“ Jars, ointment, 60-mL, squat form, with PTFElined screw caps. 4. Volumetric flasks, 10- and 100-mL 5. Pipet, 3-mL. 6. Vials (for autosampler) . 7. Liquid chromatograph with gradient capability, UV detector (254 nm) and column, (see page 5010-1) . 8. Syringes, 5-mL. 9. Syringe filters, PTFE; or Swinnex adaptor with luer fitting containing 0.5-μm PTFE filter, 13-mm. ”