National Institute for Occupational Safety and Health

“ Pipet 0.1 mL of each organotin standard solution into a 10-mL volumetric flask. Dilute to the mark with 0.1% (v/v) acetic acid in acetonitrile. Prepare fresh daily.
SPECIAL PRECAUTIONS: None. SAMPLING: 1. Calibrate each personal sampling pump with a representative sampler in line. 2. Break the ends of the sorbent tube immediately before sampling and connect to the filter with a short piece of tubing. Attach sampler to personal sampling pump with flexible tubing. 3. Sample at an accurately known flow rate between 1 and 1.5 L/min for a total sample size of 50 to 500 L. 4. ”

“ SAMPLE PREPARATION: NIOSH Manual of Analytical Methods (NMAM) , Fourth Edition, 8/15/94 Organotin Compounds (as Sn) : Method 5504, Issue 2 dated 15 August 1994 - Page 3 of 6 5. Open the cassette filter holder. With tweezers, carefully transfer the filter to a 125-mL beaker. 6. Place the front sorbent section and front glass wool plug into a second beaker. Place the back sorbent section and remaining glass wool plugs into a third beaker. 7. Pipet 10.0 mL acetonitrile and 10 μL acetic acid into each beaker. Cover with plastic film. 8. Agitate beakers in ultrasonic bath for 30 min. ”

“ The working range is 0.015 to 1 mg/m3 (as Sn) for a 300-L air sample. The method was validated using TeBT, TBTC, TCHH, and BuIOMA as surrogates for the most important classes of organotin compounds [1] . If speciation of organotin compounds is not required and if inorganic tin compounds are absent, the HPLC separation may be deleted. Use special care to avoid losses of TeBT and other volatile tetra-substituted organotin compounds INTERFERENCES: Organotin compounds not separated chromatographically will mutually interfere. ”