National Institute for Occupational Safety and Health

“ SAMPLE PREPARATION: 4. Open filter cassette; transfer filter to ointment jar. 5. Add 4.0 mL acetonitrile; gently swirl for 30 min. 6. Filter each sample using a 5-mL syringe with PTFE syringe filter or Swinney filtration device. Deliver filtrate to a 4-mL vial. CALIBRATION AND QUALITY CONTROL: 7. Prepare at least six working standards daily in the range 0.01 to 1 mg Rotenone per sample. a. Add known amounts of calibration stock solution to acetonitrile in 10-mL volumetric flasks and dilute to the mark. ”

“ Rotenone, 97% purity. Water, distilled, HPLC grade. Calibration stock solution, 3 mg/mL. Dissolve 0.075 g Rotenone in 25 mL acetonitrile. Prepare fresh daily in duplicate. Recovery stock solution, 50 mg/mL. Dissolve 0.500 g Rotenone in acetone. Dilute to 10 mL. Prepare fresh daily.
See SPECIAL PRECAUTIONS. 1. Sampler: 37-mm, two-piece cassette containing 1-μm PTFE membrane filter with backup pad. NOTE: Use an opaque cassette or otherwise shield the filter from light to minimize photodecomposition of Rotenone during and after sampling. 2. ”

“ Chemists , 58, 965 (1975) . [5] NIOSH Research Report-Development and Validation of Methods for Sampling and Analysis of Workplace Toxic Substances, U.S. Department of Health and Human Services, Publ. ”