National Institute for Occupational Safety and Health

Name: NIOSH Manual of Analytical Methods — 1-Nitropyrene in Diesel Particulates (2560)
Author: National Institute for Occupational Safety and Health

NIOSH Manual of Analytical Methods… (1994)

Summary

“NIOSH Manual of Analytical Methods — 1-Nitropyrene in Diesel Particulates (2560)”, published in 1994, is a work by National Institute for Occupational Safety and Health. It explores elements such as filter, nitropyrene and calibration.

Quotes from NIOSH Manual of Analytical Methods… (National Institute for Occupational Safety and Health)

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Average recoveries of 1-nitropyrene from spiked glass fiber filters ranged from 88.4% to 101.2% for an alyte quantities of 1.0 to 50.0 :g/filter using liquid standards as the spiking solution. Recovery of 1-nitropyrene from NIST SRM-1650 Diesel Particulates weighed on glass fiber filters and carried through the extraction and sam ple preparation process yielded recoveries of 73% to 114% (see NOTE) for sample quantities of 1.63 mg to 26.6 mg. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Inject sample with autosampler or by manual injection. NOTE: If peak are a is above the linear range of the calibra tion sta nda rds, dilute the sam ple with toluene, reanalyze, and apply the appropriate dilution factor in calculations. 10. Measure peak area of 1-nitropyrene peak and determine μg per sample from calibration graph. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Place a 37 mm glass fiber filter on top of the space r ring (do not use a supp ort pad) . b. Inoc ulate the filter with a k now n am oun t of ca libration s tock solution with a m icroliter syringe, place the top section of the cassette in place and seal the filter in place by squeezing the two sections together. c. Place the plugs in the inlet and outlet tubing stubs and set the cassettes aside for a minimum of twelve hou rs. d. Prepare the filter samples (steps 4 & 5) and analyze with the calibration standards. e. Prepare a graph of R vs. μg 1-nitropyrene. 8. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Micro volume inserts for autosampler vials. 7. Forceps. 8. Syringes, glass: 5-μL, 10-μL, 1-mL, other sizes as needed. 9. Syringe filter with tube tip, 4-mm , 0.45-μm, PVDF. 10. Syringe, disposable: 1-mL volume. 11. Gas Chrom atograph: HP-5890 with HP-7673 Au tos am pler (or eq uivalent) , An tek Model 704E N itrogen Chem ilum inescence Detector (or equivalent) . Restek Rtx-5-ms®, 30 m X 0.32 mm id., 1.0-:m film thickness (or equivalent) .
See SPECIAL PRECAUTIONS SPECIAL PRECAUTIONS:1-Nitropyrene is a cancer suspect agent and mutagen. Toluene is a flamm able liquid, toxic. SAMPLING: 1. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ None identified - however, compounds with a similar retention time may interfere. Positive identification may be confirmed by dual column chromatography using an appropriate alternative capillary column. Identification can also be confirmed by mass spectrometry. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ M ETH OD 2560, Issue 1, dated 1 5 Ma rch 2003 - Page 4 of 5 The method LOD and LOQ as determined using spiked media samples were 3.4 ng/sample and 11.5 ng/sample respectively. However, because the detector was operating at its m ax im um sen sitivity settings, the normal baseline noise at th e lowe st analyte levels often m ade the peak d ifficult to be pro perly integrated. ”

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Source: Wikisource

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ To luene, HPLC or GC grade .* 2. NIST , SRM 1650, Diesel Particulate Matter. 3. 1-Nitropyrene Standard Solution, 5000 μg/mL in toluene. (A ccu Stan dard Inc., N ew H aven, CT , or equivalen t) .* 4. 1-Nitropyrene Standard Solution, 1000 μg/mL in toluene. (A ccu Stan dard Inc., N ew H aven, CT , or equivalen t) .*
1. Sam pler: Glass fiber filter (Type A/E) 1-:m pore size, 37-mm diameter (Gelman Sciences, A nn A rbor, MI, or equ ivalent) , back ed by a cellulose support pad 37 -m m OD, in a two part, 37-mm cassette filter holder. 2. Spacer Ring, 37 mm (SKC, Eighty Four, PA or eq uivalen t) . ”

National Institute for Occupational Safety and Health

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Quotes from NIOSH Manual of Analytical Methods… (National Institute for Occupational Safety and Health)

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