National Institute for Occupational Safety and Health

“ Using a 10-μL syringe and the calibration stock solution inject four sampling tubes at each of five levels. Open several blank tubes but add no acrolein to them. Cap all the tubes. b. Using the 10-μL syringe, spike the calibration stock solution into 4-mL vials containing 2 mL of toluene and 10 mg of purified 2- (hydroxymethyl) piperidine. Prepare four replicate vials at each of the five concentrations used in step a. c. Allow the spiked tubes, spiked standard solutions and blank tubes to stand overnight at ambient temperature to assure complete reaction. ”

“ Desorb and analyze the spiked and blank tubes according to steps 4 through 11. Analyze the spiked solutions according to steps 10 and 11. d. To calculate the recovery, compare the blank-corrected peak areas or heights of the acrolein peak found in the spiked tubes (Q r - B) to the peak area or height of the acrolein peak found in the spiked solutions (Q a) . ”

“ Inject 1-μL sample aliquot. 11. Measure peak area or peak height. Acrolein derivative t r = 3.6 min and t r for 2- (hydroxymethyl) piperidine = 10.7 min for these conditions. CALCULATIONS: 12. Read the μg/mL of acrolein found in the sample front (W f) and back (W b) sorbent sections from the calibration graph and multiply by the desorption volume, 2 (mL) . Calculate concentration, C (mg/m 3) , of acrolein in the air volume sampled, V (L) : NOTE: Because the working standards are prepared on media blanks, no additional blank correction is necessary. ”