National Institute for Occupational Safety and Health

“ Calibrate daily with at least six working standards over the range 0.4 to 360 μg anisidine per sample for each isomer. a. Add a known volume of calibration stock solution, or a dilution thereof in methanol, to a 5-mL volumetric flask and dilute to the mark with methanol. b. Analyze together with samples and blanks (steps 11 and 12) . c. Prepare calibration graph (peak area vs. μg analyte) for each isomer. Determine desorption efficiency (DE) at least once for each lot of XAD-2 used for sampling in the concentration range of interest. Prepare four tubes at each of five levels. ”

“ The generation system was constructed so that samples were generated for both isomers at the same time. Concentrations were verified by an independent method using bubblers containing methanol and HPLC analysis [1] . The overall precision, SˆrT, for the combined sampling and measurement of both isomers was 0.068 with an average recovery of 100.7% for the o-isomer and 99.7% for the p-isomer [1] , which represent non-significant biases for the isomers. A separate breakthrough test was conducted for each isomer with XAD-2. ”

“ Anisidine can irritate the skin. Methanol and anisidine can be absorbed through the skin. Avoid inhalation of vapors of these compounds and of acetonitrile [4] .
SAMPLING: 1. 2. 3. 4.
Calibrate each personal sampling pump with a representative sampler in line. Uncap the sampler immediately before sampling. Attach sampler to personal sampling pump with flexible tubing. Sample at an accurately known flow rate between 0.5 and 1.0 L/min for a total sample size of 24 to 320 L. Do not sample at less than 0.5 L/min. ”