National Institute for Occupational Safety and Health

Name: NIOSH Manual of Analytical Methods — Ethylene Oxide (1614)
Author: National Institute for Occupational Safety and Health

NIOSH Manual of Analytical Methods… (1994)

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“NIOSH Manual of Analytical Methods — Ethylene Oxide (1614)”, published in 1994, is a work by National Institute for Occupational Safety and Health. It explores elements such as sample, flask and mL.

Quotes from NIOSH Manual of Analytical Methods… (National Institute for Occupational Safety and Health)

National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ STANDARDIZATION OF ETHYLENE OXIDE STOCK SOLUTION [7] 1. 2. 3.
4. 5. 6. 7.
Pipet 20.0 mL standardized HCl-pyridine solution into a 100-mL round bottom flask. Add 2.0 mL EtO stock solution. Attach the flask to the condenser. Reflux for 20 min. NOTE: The reaction is: EtO + HCl (xs) chloroethanol. The excess HCl is back-titrated in step D-6. Cool to near room temperature. Add 5 mL distilled H 2O through the condenser, collecting washings in the flask. Remove the condenser and add a drop of phenolphthalein solution to the flask. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ PREPARATION OF SOLID SORBENT Slowly add a mixture of 25 mL 48% hydrobromic acid and 125 mL acetonitrile to 75 g petroleum-based charcoal (SKC Inc., Lot 208) contained in a 500-mL round-bottom flask. Allow the slurry to cool to room temperature. Dry the coated charcoal by rotary evaporation using gentle heat and keep it overnight under vacuum at ambient temperature. The product is stable for four months when stored in a tightly sealed amber glass jar at room temperature [1] . Recovery may be lower as the sample medium ages. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ ESTIMATED LOD: 1 μg EtO per sample [2] PRECISION ( r) :
0.020 @ 18 to 71 μg EtO per sample [1]
0.062 [1] ±19%
APPLICABILITY: The working range is 0.05 to 4.6 ppm (0.08 to 8.3 mg/m3) for a 24-L air sample. The method is applicable to short-term (10-min) samples.
INTERFERENCES: 2-Bromoethanol, if present in the sample interferes. No other significant interferences have been found [1] .
OTHER METHODS: This is a modification of OSHA Method 50 [1] and replaces NIOSH method 1607, which has smaller sample capacity, and S286 [3] , which is useful for higher levels. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ The 5% breakthrough volume for sampling a 16-ppm atmosphere of EtO at 0.15 L/min was 39 L. No significant storage effects were observed for samples in the 0.1 to 16 ppm range at high humidity and stored at ambient temperature for a minimum of two weeks. The precision, Sr, of chromatographic response of working standards in the range 18 to 71 μg EtO per sample was 0.028 [1] . Recovery of EtO spikes in NIOSH laboratories averaged 75% at 11, 22, 33, and 44 μg EtO per sample. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Mix 1 min to ensure complete hydrolysis of excess HFBl. Transfer at least 1.0 mL of isooctane (top) layer to 2-mL vial. NOTE: If the sample concentration is higher than the standards, dilute an aliquot of sample with DMF, reanalyze starting at step 9, and apply the appropriate dilution factor in calculations. The desorbed sample (in DMF) keeps well in a freezer [1] .
CALIBRATION AND QUALITY CONTROL: 13.
Calibrate daily with at least six working standards over the range 1 to 42 μg EtO per sample. For example: a. Pipet 1.0 mL 2-BrEt (density 1.763 g/mL @ 20 C) into a 10-mL volumetric flask. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Analyze working standards together with samples and blanks. d. Prepare calibration graph (μg EtO per sample vs. peak area) . Use a nonlinear (e.g., NIOSH Manual of Analytical Methods (NMAM) , Fourth Edition, 8/15/94 ETHYLENE OXIDE: METHOD 1614, Issue 2, dated 15 August 1994 - Page 4 of 6
14.
15.
parabolic) least squares fit if necessary to obtain the best fit of the data. Determine recovery (R) at least once for each lot of charcoal used for sampling in the concentration range of interest. Prepare three tubes at each concentration of interest plus three media blanks. a. ”

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National Institute for Occupational Safety and Health, NIOSH Manual of Analytical Methods… (1994)

“ Water, high purity (e.g., deionized distilled) . 6. 2-Bromoethanol (2-FrEt) , 95 to 98%. 7. Sodium hydroxide (NaOH) , reagent grade. 8. Potassium hydrogen phthalate (KHP) , reagent grade, dried at 90 C. 9. ”

National Institute for Occupational Safety and Health

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