National Institute for Occupational Safety and Health

“ Pipet a 5-mL aliquot of urine into a clean culture tube. 7. Adjust pH to >12 using 10 N NaOH (ca. 0.1 mL) . 8. Add 1 mL methanol. Mix well. 9. Add 5 mL 1:1 diethyl ether:hexane. Shake steadily for 2 min to extract the MBOCA. Centrifuge to separate phases. Transfer the organic phase by Pasteur pipet to a culture tube. Repeat with two additional 5-mL portions of 1:1 diethyl ether: hexane, combining the organic phases. NOTE: Emulsions may form; break them by adding methanol or by repeated centrifugation. 10. Concentrate the extract to ca. 1 mL under a gentle stream of nitrogen in a hood. ”

“ Volumetric flasks, 100- and 250-mL. 16. Glass wool.
See SPECIAL PRECAUTIONS.
SPECIAL PRECAUTIONS: Samples of urine collected from humans pose a real health risk to laboratory workers who collect and handle these samples. These risks are primarily due to personal contact with infective biological samples and can have serious health consequences, such as infectious hepatitis, and other diseases. There is also some risk from the chemical content of these samples, but this is much less. Those who handle urine specimens should wear protective gloves, and avoid aerosolization of the samples. ”

“ Mouth pipetting, of course, must be avoided. MBOCA, MDA, and benzene are suspect carcinogens; handle either in a glove box or a well-ventilated hood. Handle ethyl ether and hexane with care; both are highly flammable and should be used in a hood. ”