National Institute for Occupational Safety and Health

“ Determine the mass (mg) of nicotine (corrected for DE) found in the sample front (W f) and back (W b) sorbent sections and in the average media blank front (B f) and back (B b) sorbent sections. NOTE: If W b ≥ W f/10, report breakthrough and possible sample loss. Calculate the concentration of nicotine in the actual air volume V (L) at the sampling site:
EVALUATION OF METHOD: Method S293 [2] was issued 3/26/76. Synthetic atmospheres of the analyte in dry air were generated dynamically at 22.1 to 24.5 °C and 765.4 to 767.9 torr (102 to 102.4 kPa) over the range 0.3 to 1.2 mg/m 3. ”

“ Calibrate daily with at least six working standards covering the analytical range 0.005 to 0.13 mg nicotine per sample. a. Add 1 to 25 μL or other convenient aliquots of the calibration solution to 1.0 mL of ethyl acetate in separate vials. Correct the volume of ethyl acetate in each vial to account for volume of the aliquot of calibration solution added. Seal the vials with septum-lined crimpcaps. b. Analyze the standards together with the samples (steps 11 through 13) . c. Prepare a calibration graph of peak area vs. mg of analyte. ”

“ Nicotine is a SAMPLING: vasoconstrictor. Perform all work with these chemicals in a hood. 1. Calibrate each personal sampling pump with a representative sampler in line. 2. Before sampling, score the ends of each sampling tube with a file, and break open. Attach the sampler to personal sampling pump with flexible tubing. 3. Sample at 1.0 L/min to obtain the recommended sample volume of 60 to 400 L. 4. Cap the tubes with plastic (not rubber) caps immediately following sampling and pack securely for shipment. ”