National Institute for Occupational Safety and Health

“ Prepare at least 6 working standards in the range 0.01 to 1 μg/mL PCP by dilution of the calibration stock solution with hexane. Prepare a calibration graph (peak area vs. μg/mL PCP) . Maintain standardization by injecting a working standard after each five sample injections. Run a spiked pooled control blood sample every ten samples, or if less than ten determinations are required, run at least three spiked pooled blood samples per study. NOTE: All blood that will be utilized for spiked pooled control blood samples must be analyzed for PCP before use because PCP is ubiquitous. ”

“ These risks are primarily due to personal contact with infective biological samples and can have serious health consequences, such as infectious hepatitis, and other diseases. There is also some risk from the chemical content of these samples, but this is much less. Those who handle blood specimens should wear protective gloves, and avoid aerosolization of the samples. ”

“ Centrifuge (refrigerated, optional) . 6. Chromatography column, 7-mm ID x 200 mm, with a 50-mL reservoir and PTFE stopcock.** 7. Syringe, 10-μL, glass. 8. Pipets, 10- to 1000-μL.** 9. Flasks, volumetric, 10-, 25-, and 100-mL.** 10. Pipets, Pasteur, disposable. 11. Tubes, centrifuge, graduated, glass-stoppered, 15-mL.** 12. Waterbath, 100 °C.
Wash with detergent; clean with chromic acid; rinse with distilled water, acetone, and hexane.
SPECIAL PRECAUTIONS: Samples of blood collected from humans pose a real health risk to laboratory workers who collect and handle these samples. ”