National Institute for Occupational Safety and Health

“ Determine the mass, μg (corrected for R) of Ribavirin found in the sample (W) , and in the average media blank (B) , from the calibration graph. Calculate concentration, C, of Ribavirin in the air volume sampled, V (L) :
EVALUATION OF METHOD: This method is a modification of a bulk assay procedure developed by Eastman Kodak Company [3] . Measurement precision, Sr, was 0.057 with average recovery of 100% representing no bias, based on 16 samples ranging from 19.2 to 112 μg per filter. Sampling precision was not determined. ”

“ Ribavirin has been found to be teratogenic in animals. [4, 5] Use protective gloves when handling. Work only in a fume hood. Women of childbearing age should exercise extreme caution. Avoid skin contact with concentrated sulfuric acid.
SAMPLING: 1. 2. 3. 4.
Calibrate each personal sampling pump with a representative sampler in line. Attach sampler to personal sampling pump with flexible tubing. Sample at an accurately known flow rate between 1 and 4 L/min for a total sample size of 5 to 1000 L. Avoid overloading the filter (ca. 2 mg total dust maximum loading) . ”

“ UV detector (210 nm) ; peak integrator; and cation exchange resin column (Cat. No. HPX-87H, Bio-Rad Laboratories, Richmond, CA 94804 or equivalent) . 4. Vials, 10-mL, glass with PTFE-lined caps. 5. Culture tubes, PTFE-lined screw caps, 13-mm x 100-mm. 6. ”