National Institute for Occupational Safety and Health

“ Prepare graphs of R vs. amounts found. (2) Sorbent tubes. Transfer an unused front sorbent section to a culture tube. Prepare a total of 24 culture tubes in order to measure DE at five concentration levels plus blank in quadruplicate. Using a microliter syringe or micropipette, add calibration stock solution directly to sorbent. Cap culture tubes and allow to stand overnight. Desorb and analyze (steps 8, 9, and 13 through 15) . Prepare graphs of DE vs. amounts found. b. Check R and DE at two levels for each sample set, in duplicate. ”

“ Counter tops and equipment should be checked regularly with a "black light" for fluorescence as an indicator of contamination by PAHs.
NIOSH Manual of Analytical Methods (NMAM) , Fourth Edition POLYNUCLEAR AROMATIC HYDROCARBONS by HPLC: METHOD 5506, Issue 3, dated 15 January 1998 - Page 3 of 9
SAMPLING: 1. Calibrate each personal sampling pump with a representative sampler in line. 2. Take personal samples at 2 L/min for a total sample size of 200 to 1000 L. 3. Immediately after sampling, transfer the filter carefully with forceps to a culture tube. ”

“ If sampling is to be done in bright sunlight, use opaque or foil-wrapped cassettes to prevent sample degradation. b. Sorbent tube, washed XAD-2 resin (front = 100 mg; back = 50 mg) (ORBO 43, Supelco, Cat. No. 2-0258; or equivalent) , connected to filter with minimum length of PVC tubing. Plastic caps are required after sampling. NOTE: If pressure drop is excessive or pump fails, use a larger diameter sorbent tube with XAD-2 resin (ORBO 42 Large, Supelco, Cat. No. 2-0264U; or equivalent) . 2. Personal sampling pump capable of operating for 8 h at 2 L/min, with flexible connecting tubing. 3. ”