National Institute for Occupational Safety and Health

“ Standard precautions should always be used when handling bodily fluids and/or extracts of bodily fluids [15] . Handle urine samples and urine extracts using proper gloves. Acetone and MEK are highly flammable liquids. All work should be performed in a fume hood since both chemicals are respiratory irritants and may depress the central nervous system at high exposure levels. SAMPLING: 1.
Collect urine in a 125-mL polyethylene bottle or other suitable container. Collect at least two urine samples from each worker. Collect one sample before exposure and one sample after exposure. ”

“ Process the 10 mL of each working standard and each pooled urine blank using the same procedure as for the samples (steps 5 through 8) . e. Analyze the working standards, the pooled urine blanks, and the samples together. FIGURES 1 and 2 show representative chromatograms of blank and fortified urines. f. Prepare a calibration graph by plotting, for each working standard, the normalized analyte response (peak area of analyte divided by the peak area of the internal standard on the same chromatogram) on the y-axis vs. μg of analyte/mL of urine on the x-axis. ”

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