National Institute for Occupational Safety and Health

“ Robustness: Multiple HP-1 (100% dimethylpolysiloxane) columns of different manufacturing lots were used during the recovery studies. Accuracy and precision did not appear to be affected; therefore, the method appears to be reproducible with any normal functioning HP-1 capillary column. Recovery results from individual urine samples spiked with 3-BPA indicate that the method is accurate and not significantly affected by individual urine sample matrix differences during analyte extraction. ”

“ Both recovery studies generated a total of 47 spiked urine samples at 2, 10, 20 and 50 μg/mL 3-BPA levels. Precision expressed as percent relative deviation (% RSD) was as high as 5.7% on the 2 μg/mL recovery samples (n = 9) . Overall recovery was 95% and overall RSD was 3.1% (n = 47) . Linearity: All calibration curves used during the development of this method were linear and had correlation coefficients of 0.98 and greater. The concentration range was 0.1 to 200 μg/mL 3-BPA in urine with 2.0 mL urine sample size. Calibration curves were run at the beginning and end of all sample batch runs ”

“ Note: Because exposure limits and guidelines may change over time, NIOSH recommends referring to the following sources for updated limits and guidelines on the use of this compound as a marker for 1-bromopropane [1,2,3,4] . ”

“ Collect at least 15 mL urine in an appropriate polypropylene tube or bottle and cap. Refrigerate or freeze immediately after collection. Collect at least two urine specimens for each worker: one before the work shift and one after. 2.
Ship the specimens stored in either wet or dry ice in an insulated container. Store frozen upon arrival at the laboratory. A reminder: commercial shippers have special labeling requirements for packages containing dry ice. SAMPLE PREPARATION: 3.
Thaw the urine specimen to room temperature. 4.
Mix thoroughly to ensure urine homogeneity. 5. ”

“ The oxidative metabolism of 1-bromopropane in the rat. Xenobiotica 9 (12) : 763-772. [8] Mathias PI, Cheever KL, Hanley KW, Marlow KL, Johnson BC, B’Hymer C [2012] . Comparison and evaluation of urinary biomarkers for occupational exposure to spray adhesives containing 1-bromopropane. Toxicol Mech Methods 22 (7) : 526-532. [9] B’Hymer C, Cheever KL [2004] . Development of a gas chromatographic test for the quantification of the biomarker 3-bromopropionic acid in human urine. ”