National Institute for Occupational Safety and Health

“ NIOSH Manual of Analytical Methods (NMAM) , Fourth Edition ANILINE and @-TO LUIDIN E in urine: MET HO D 831 7, Issue 1, dated 15 M arch 200 3 - Page 3 o f 6 SAMPLING: 1. Timing of Collection: In humans, aniline is rapidly metabolized and excreted in the urine [1] . Rat data sug ges t that @-toluidine likewise is rapidly metabolized and excreted [2] . Thus, biological monitoring using pre and post shift urine samples is recomm ended in order to capture the metabolites quickly after exposure. 2. Collect urine in one or more 250-mL polypropylene bottles. ”

“ Make the nominal mass of amine in pg injected the independent X variable and its peak height response the dependent variable of the calibration curve.
whe re a, b, and c are regress ion co efficients 22. Also, prepare quality control samples by collecting 1 L of fresh urine from unexposed non-smoking individuals, who are not taking medication. 23. To one liter of this fresh urine add 100 g of citric acid. 24. Add 25, 5, and 1 mL of stock 1000-μg/L standard solution to three 250-mL volumetric flasks, and dilute to the mark with the acidified urine. ”

“ The Karam El-Bayoumy et al. study [3] reported the total mass of aniline and @-toluidine excreted by urine of 19 occ upa tionally exp ose d su bjec ts, and they found that the work ers exc reted from 0.02 to 8.8 :g of aniline and 0.3 to 12.9 :g of @-toluidine during a 24-hour period.
EVALUATION OF METHOD: The American Conference of Governmental Industrial Hygienists has recom m ended a biological expos ure index (BEI) for aniline of 50 mg of 4-aminophenol per milligram of creatinine, measured in end-of-shift urine specimens. ”