National Institute for Occupational Safety and Health

“ Prepare glutaraldehyde oxazolidine standard solutions. a. Add known amounts of glutaraldehyde oxazolidine stock solution (equivalent to the range of the samples) to toluene in 10-mL volumetric flasks and dilute to the mark. b. Analyze (steps 12 and 13) with samples and blanks for qualitative identification of derivative peaks. Calibrate daily with at least five working standards covering the range of the samples. a. Weigh 120-mg portions of unused sorbent from media blanks into vials. b. Add aliquiots of glutaraldehyde stock solution or dilutions thereof. ”

“ Relative humidity during generation was controlled at 80% ±5%. The generator and sampling manifold systems have been described previously [4] . Concentration of glutaraldehyde vapor was independently verified by the 2,4-dinitrophenylhydrazine procedure of Lipari and Swarin [5] . No bias with dynamicallygenerated atmospheres was observed with the method over the range 0.8 to 8 mg/m 3 using 22-L air samples. Desorption efficiencies on statically-spiked samples averaged 87% in the ranges 5 to 50 μg/sample. ”

“ H 2O GLUTARALDEHYDE: METHOD 2531, Issue 2, dated 15 August 1993 - Page 3 of 5 SAMPLE PREPARATION: 4. 5. 6. 7. 8.
Score each sampler with a file in back of the back sorbent section. Break sampler at score line. Remove and place back glass wool plug and back sorbent section in a vial. Transfer front section with remaining glass wool plugs to a second vial. Add 2.0 mL toluene to each vial. Screw cap tightly onto each vial. Agitate vials in an ultrasonic bath for 60 min. NOTE: Desorption efficiency is affected by the amount of time that the vials are allowed to spend in the ultrasonic bath. ”