National Institute for Occupational Safety and Health

“ Filter the sample solution through a syringe filter.
CALIBRATION AND QUALITY CONTROL: 6.
7.
Calibrate daily with at least six working standards over the range 1 to 70 μg strychnine per sample (0.2 to 14 μg/mL) . a. Add known amounts of calibration stock solution to mobile phase in 10-mL volumetric flasks and dilute to the mark. b. Analyze together with the samples and blanks (steps 9, 10, and 11) . c. Prepare calibration graph (peak area vs. μg strychnine) . Determine recovery (R) at least once for each lot of filters used for sampling in the calibration range. ”

“ SPECIAL PRECAUTIONS: Strychnine is a potent convulsant. Use impervious clothing, gloves and face shields when handling it [3,4] . Avoid skin contact with acetic acid as it may cause severe burns.
SAMPLING: 1. 2.
Calibrate each personal sampling pump with a representative sampler in line. Sample at an accurately known flow rate between 1 and 3 L/min for a total sample size of 70 to 1000 L.
SAMPLE PREPARATION: 3. 4. 5.
Transfer the filter carefully using tweezers to an ointment jar. Add 5.0 mL mobile phase. Seal and gently swirl the jar to wet the filter. ”

“ NIOSH/OSHA Occupational Health Guidelines for Chemical Hazards, U.S. Department of health and Human Services, Publ. (NIOSH) 81-123 (1981) , available as GPO Stock #017-033-00337-8 from Superintendent of Documents, Washington, D.C. 20402. NIOSH Research Report - Development and Validation of Methods for Sampling and analysis of Workplace and Toxic Substances, U.S. Department of Health and Human Services, Publ. ”