Frederic A. Woll

“ To study the lens properly one should use an eye that has not been hardened and also an eye or the lens of an eye that has been in a 5 per cent. solution of formaldehyde for about two weeks.
The lens in the unhardened eye will prove too friable to permit much handling. The dissection should be made, however, in order to give opportunity to notice the crystalline clearness of the lens substance, its great magnifying power, its attachments, its capsule, etc. For this purpose it is necessary to proceed only as in the dissection for the “hyaloid membrane, etc.” ”

“ Procure the eye of either a sheep or a bullock. Instead of following the usual procedure of hardening in any one of the several solutions used for the purpose of toughening the ocular tissues, place the eye in a cool place and permit it to collapse a trifle. Usually two or three days is a sufficient length of time to accomplish the result.
Experiments have shown that if an eye is [27] too fresh the ciliary processes will not be easily detached from the hyaloid (zonular processes) , and if the eye has been in a preserving fluid, the same result will follow. ”

“ The eyes one procures from a butcher or a slaughter house will always have the extrinsic tissues so badly cut and torn that identification of the various parts and their relations is impossible. Therefore, it is best to supply one’s self with the head of an animal, such as a sheep or a calf, and dissect an eye with all its extrinsic tissues intact. For this dissection, a hammer and a chisel are necessary in addition to the tools needed for doing the previous dissections. ”

“ Still another way to see the interior is to cut out a piece of the sclerotic about the size of a twenty-five-cent piece; then pinch up and tear out the choroid and the retina under the opening made in the sclerotic. (Fig. 57.) Hold the eye, the cornea forward, close to a bright light, and the image of the light will be seen upon the retina. The closer the light is to the eye, the greater the illumination will be in the [111] interior of the eye. If the opening or “window” is close enough to the optic nerve, the optic papilla can be seen easily. ”

“ For the study of the interior of the eye and its contents in situ either a fresh or a hardened eye will do; a hardened eye is preferable. In the dissection for isolating the hyaloid membrane, vitreous, lens, and other parts, the anterior and posterior halves of the evacuated eye may be separated entirely, and each half studied. However, the choroid and the retina will be more or less mutilated, and the vitreous and other parts will be removed. ”

“ One reason why eye dissections are easily carried on is because material can always be [20] readily procured. Any butcher will furnish sheep, pig, or beef eyes. Or, if one has the time to visit the manager of a slaughter-house, and make known to him one’s needs, he will supply enough eyes to carry through a host of interesting dissections and experiments, and give sufficient material for careful, orderly, and fruitful study. ”

“ To see the iris, take hold of the cut edge of the choroid, and, gently pulling, separate it from its attachment to the corneo-scleral junction. The white ring on the anterior surface of this part of the second coat of the eye is the ciliary ring. With a scissors, cut around this ciliary ring at its outer edge. This specimen will show the anterior surface of the iris, and on the posterior side it will show the close relationship between the iris and the ciliary [52] processes. A hand lens will help greatly to bring out the very interesting fine points. ”

“ In order of importance, patience really precedes dexterity, skill, and observation; and persistence of effort is a factor not to be entirely outshone by any other virtue. With these attributes, knowledge of the subject in hand naturally follows. ”

“ Care must be taken not to lacerate the anterior surface of the lens when the iris is drawn back by the pins.
Putting the pins into the cornea, and using them as levers with the point of entrance in the cornea as a fulcrum, will pucker the cornea considerably, and a good clear fundus cannot be obtained. This is easily overcome. Simply gather up all the tissues surrounding the eye, force them backward, and hold them firmly with the fingers of the left hand. ”

“ An examination of the eyelids will show the openings of the ducts of the meibomian glands a short distance back of the cilia. Very fine pins or needles that have been greased may be easily inserted for a short distance into the ducts, and then a dissection made along the course of the duct as outlined by the presence of the inserted pins or needles. Another way to see the glands is to slice through the ducts, with the scalpel or safety-razor blade, the entire width of either eyelid. ”

“ The gland lies directly over the eye and near the outer angle of the orbit. In the enucleated eye, it will be found to lie near the outer canthus and over the eye. The gland may be easily dissected out of its position and then examined more closely. A hand lens will show the racemose construction of the gland. If the gland is cut in two, the racemose construction may be seen even better. ”

“ Though there is a difference between the eyes of human beings and the eyes of other animals, the difference is slight and of minor importance when compared with the similarity of the more important parts. ”

“ Isolating the retina from the other tissues requires considerable patience and dexterity. When the retina has been removed and placed in a special receptacle, it will be found that the specimen is well worth the little amount of time spent in making it. ”

“ It will be well to state here that during this dissection the specimen should not be lifted from the table. Keep the eye resting on the table all the time, and never lift it by holding it suspended from the optic nerve. [67] Loosening the choroid from the sclerotic up to this point is a very easy matter; ordinary precaution is all that is necessary to prevent puncturing the choroid with the scalpel, just be sure to remember to keep the point of the scalpel close to the sclerotic. ”

“ Another good specimen may be made by removing all of the inner tissues, and leaving only the sclerotic and cornea. This specimen will show that the first coat is almost entirely a coat which affords strength and protection to the parts that lie within.
[92]
THE PAPILLA, PUNCTA LACRIMALIA, AND NASAL DUCT
The only way to dissect the lacrimal apparatus, other ocular accessories, and the extrinsic muscles, is to procure the head of some animal, preferably a calf’s head, because of its size. Any butcher will supply one for from forty to sixty cents. Have the lower jaw removed. ”

“ Insert the needle into the punctum of the [93] lower lid, and push it downward and forward, aiming to come out in the nose a short distance from its end. At first it may be a little difficult to get the needle started; if so, just wiggle the needle, pushing it at the same time as directed, until the nasal duct is found. ”