Nitrogen fixation

“ We have no very absolute knowledge as to the agency of bacteria in furnishing nitrogen for this vegetation in past ages, but there is every reason to believe that in the past, as in the present, the chief source of organic nitrogen has been from the atmosphere and derived from the atmosphere through the agency of bacteria. In the absence of any other known factor we may be pretty safe in the assumption that bacteria played an important part in this nitrogen fixation, and that bacteria must therefore be regarded as the agents which have furnished us the nitrogen stored in the coal. ”

“ Schramm in America, working with pure cultures of algæ, tried for ten years to establish the fact of nitrogen fixation, and failed completely; more recently Wann has extended Schramm’s work, and claims to have proved indisputably that, given media containing nitrates as a source of nitrogen and a small amount of glucose, the seven species of algæ tested by him fixed atmospheric nitrogen to the extent of 4-54 per cent. of the original nitrogen content of the medium. ”

“ The gaseous nitrogen in the soil is also of some importance, since it is in this material that the anaerobic organisms which accumulate on the rootlets of some plants probably act in the process of the fixation of atmospheric nitrogen in a 13form accessible to plants. Nitrogen in the free state, it is believed, is not directly absorbed into the tissues of plants. It is necessary that it be oxidized in some way to nitric acid before it can be assimilated. The importance of nitrogen as a plant food can not be too highly estimated. ”

“ But it has been found that certain classes of plants (the Leguminosæ, for example) , when they are grown in a soil which is practically free from nitrogen at the commencement, do take up this gas into their tissues. One explanation of this fact is that free nitrogen becomes converted into nitrogen compounds in the soil through the influence of micro-organisms present there. Another explanation attributes this fixation of free nitrogen to micro-organisms existing in the rootlets of the plant. ”

“ Additional evidence is given to show that the concentration in nitrates in brown spots in which nothing will grow is not due to the accumulation of preexisting nitrates, but to the action of microorganisms which are able to bring about the fixation in the soil of atmospheric nitrogen, and that the dark-brown color which is characteristic of the spots is due, not to black alkali, but to the development of pigment by the organisms. ”

“ But it is also possible that some direct oxidation of protein or amino-acids may yield gaseous nitrogen. However it is brought about it affects a considerable proportion of the entire stock of nitrogen, and it becomes more serious as cultivation is intensified. ”

“ Nascent hydrogen is a powerful reducing agent, and would of itself withdraw the oxygen from nitrates to form nitrites, while hydroxyl in contact with ammonia will cause the formation of water and the liberation of nitrogen. Part of this nitrogen is undoubtedly utilized by the bacteria, and the rest is returned to the air. How the inert nitrogen molecule is torn apart and the nascent nitrogen atoms thus formed utilized by the bacteria for their growth is a question that is more difficult of solution. ”

“ When a bit of meat decays, part of the meat is, indeed, converted into ammonia or other nitrogen compounds, but if the putrefaction is allowed to go on, in the end a considerable portion of it will be broken into still simpler forms, and the nitrogen will finally be dissipated into the air in the form of free nitrogen. This dissipation of free nitrogen into the air is going on in the world wherever putrefaction takes place. Wherever decomposition of nitrogen products occurs some free nitrogen is eliminated. ”

“ As regards the first alternative, it must be stated that we know of no source other than the decomposition of plants from which ammonia can be derived, and we are therefore compelled to adopt the second alternative, and to admit that there must be some other source of nitrogen, and it cannot be doubted, from what has been already stated, that it is from nitric acid only that it can be obtained. ”

“ When plant and animal products are changed into soluble nitrates, they are usually soon lost to the soil, unless taken up by the roots of plants. When vegetable matter on or near the surface of the ground is broken down and decomposed, in the process of oxidation, there is frequently much loss of nitrogen, as in the rapid decomposition of farmyard manure in the absence of some material, as land plaster, to arrest and hold the escaping ammonia. ”

“ This is most cheaply done by neutralizing the nitric acid with lime or the ammonia with sulfuric acid, yielding calcium nitrate or ammonium sulfate, respectively. Whether the nitrate or the ammonium salt is made the constituent of the fertilizer makes little difference; for, though plants directly assimilate the nitrogen only in the form of nitrate, yet there are always present in soils the so-called nitrifying bacteria, whose function it is to convert ammonium compounds into nitrates. ”

“ The nitric acid, as it becomes free, gives a part of its oxygen to the phosphorous compound, and the nitrous acid, in a nascent state, is at once reduced by the ammonium chlorid. There are two sources of error which must be guarded against in the work; a portion of the nitrogen may escape reduction to the elementary state, or some of the nitrate may fail to be decomposed. These errors are easily avoided if the reaction be begun slowly, so that the evolution of gas may be gradual. ”

“ A variety of nitrogenous substances have proved susceptible to nitrification in solution. The weight of evidence, however, appears to show that in all cases the nitrogen first assumes the form of ammonia, and that the latter is, strictly speaking, the only substance capable of being nitrified. ”

“ If air have free access, putrefaction (decomposition) may go on without any odour at [Pg 63] all, the evil-smelling gases (NH3 and SH2, for example) being oxidised at once to form nitrates and sulphates. Aerobic bacteria, too, such as the nitre and sulphur bacteria, bring about this mineralisation of organic nitrogen. Moreover, when air is circulating freely, there is no accumulation of intermediate products such as skatol or indol. It occurs on the surface of manure heaps, on the outer surfaces of carcases, and in well ventilated soil. ”

“ This is the general process of nitrification in which the nitrifying bacteria transform or transfer the nitrogen from insoluble organic compounds into soluble compounds in which it may serve as available plant food. The nitrate which is thus formed may be calcium nitrate or magnesium nitrate or potassium nitrate or even sodium nitrate, depending upon which of these alkaline elements is present in the must suitable form. If no alkaline element is present in available form then no nitrates can be made in the soil. ”

“ The circumstance that certain soil organisms—bacteria, algæ, and fungi—themselves assimilate ammonia and nitrate may account for the remarkable slowness of nitrate accumulation, to which reference has already been made. The protein formed from the assimilated nitrogen remains in the bodies of the organisms, living or dead, till decomposition sets in. It is not difficult to picture a cycle of events in which much of the nitrate formed is at once reabsorbed by other organisms, and only little is actually thrown off into the soil. ”

“ There are certain compounds containing nitrogen which are indispensable to our food. An animal nourished entirely on food which is destitute of nitrogen would become weak and would soon die."
"Excuse me, Monsieur Roger," said Albert Dalize: "how can nitrogen enter into our food?"
"That is a very good question," added Miette, laughing; "surely you cannot eat nitrogen and you cannot eat gas."
"The question is indeed a very sensible one," answered Monsieur Roger; "but this is how nitrogen enters into our food. We are carnivorous, are we not? we eat meat and flesh of animals. ”

“ Nitric acid is one of the strongest known, and in solvent power it is excelled by no single acid. The nitrogen contained in crop residues and other organic manures is chiefly in chemical combination with carbon, oxygen and hydrogen, much of it in insoluble protein compounds. Normally this organic nitrogen is transformed in the soil, first into ammonia nitrogen, next into nitrite nitrogen, and lastly into nitrate nitrogen, these three transformations being effected by biochemical action produced by different kinds of living microscopic organisms called bacteria. ”

“ The nitrifying bacteria are those which have the power to form nitrates. In the following brief discussion of this subject we include at least three species of bacteria which by their combined or successive action have the power to transform organic nitrogen into nitrate nitrogen, which is a suitable form of nitrogen for plant food. ”

“ In the animal body just the reverse of this process takes place,—the protein of the food undergoes a series of changes, and is finally eliminated from the body as an amid, which in turn undergoes oxidation and nitrification, and is converted into nitrites, nitrates, and ammonium salts. These forms of nitrogen are then ready to begin again in plant and animal bodies the same cycle of changes. Thus it is that nitrogen may enter a number of times into the composition of plant and animal tissues. Nature is very economical in her use of this element. ”

“ For after the mephitic portion is absorbed by a caustic alkaline lixivium, the remaining portion is not rendered salubrious; and although it occasions no precipitate in lime-water, it nevertheless extinguishes flame and destroys life." Such is the doctor's account of the discovery of nitrogen, which may be separated from the oxygen in the air in a very simple manner. The atmosphere is the great storehouse of nitrogen, and four-fifths of its prodigious volume consist of this element. ”

“ Such a case would be presented for instance, in that of a fertilizer containing dried blood, sodium nitrate, and ammonium sulfate. It is evident here that the total nitrogen could be determined by the volumetric method by combustion with copper oxid, or by the moist combustion process adapted to nitric nitrogen, but the method of determining the percentage of each constituent has not yet been described.
We have to deal here with a case entirely similar to that of phosphoric acid in a superphosphate. There is no doubt whatever of the uneven assimilability of the different forms of nitrogen. ”

“ It becomes in the soil the prey of various microbes. Ammonia appears immediately as a product of the disruption of the nitrogen-containing organic molecule. Later, oxidation processes take place, and the ammonia gives rise to nitrates which are absorbed by plants. These two processes go on successively rather than simultaneously, so that it is only towards the end of the decomposition of the organic matter that nitrification of the ammonia which is formed is set up. ”

“ It is much more probable, however, that it depends on small quantities of ammonia or nitric acid which had not been completely removed from the air by the means employed for that purpose, for nothing is more difficult than the complete abstraction of these substances, and as the gain of nitrogen was only 0·8 grains, while 60,000 gallons of air, and 13 of water, were employed in the experiment, which lasted for a considerable time, it is reasonable to suppose that a sufficient quantity may have remained to produce this trifling increase. ”

“ In general, far the largest part of soil nitrogen, excluding the atmosphere diffused in the pores of the soil, is found in the organic state and is derived from the débris of animal and vegetable life and from added fertilizers. As ammonia, the nitrogen can only be regarded as in a transition state, arising from the processes of decay, or incomplete nitrification. As nitric acid, it is found as a completed product of nitrification, or as the result of electrical action. ”

“ After the organic nitrogen of decomposing substances has been converted into ammonia, and to a small extent into free nitrogen, the latter can at once be utilised by the root-nodule organisms and other bacteria in the soil, but the ammonia must undergo two further changes and combine with a base to form a nitric salt before it is available for plant life. ”

“ The organic nitrogen may be in a form easily nitrified and rendered available for plant food, or it may be inert and resistant to nitrification, as in humus, or exist in an amid state. The ammoniacal nitrogen may exist in small quantities as gaseous ammonia, or be combined with mineral or organic acids. As nitric or nitrous acid the nitrogen will be found combined with bases, or perhaps in minute quantities as 421free acid, in passing under the influence of the nitrifying ferment from the organic to the inorganic state. ”

“ The parts of nitrogen obtained, in the form of nitrates, nitrites, and ammonia, less .032 part of 100,000 for that portion in rain, is that nitrogen derived from animal matter. Animal matters dissolved in water, such as those contained in sewage, the contents of privies and cess-pools, or farm-yard manure, undergo oxidation in lakes, rivers and streams very slowly, but, in the pores of an open soil, very rapidly. When this oxidation is complete, they are resolved into mineral compounds; their carbon is converted into carbonic acid; and their hydrogen into water ”

“ Does the skin excrete nitrogenous matter that may be, like urea, a product of the degradation or destruction of muscular tissue?
The following passage from Lehmann’s ‘Physiological Chemistry’ (vol. ii. p. 389) , shows that the skin throws out plenty of nitrogen obtained from somewhere: ‘It has been shown by the experiments of Milly, Jurine, Ingenhouss, Spallanzani, Abernethy, Barruel, and Collard di Martigny, that gases, and especially carbonic acid and nitrogen, are likewise exhaled with the liquid secretion of the sudiparious glands. ”