Reticule

“ When a little fresh blood is placed under the microscope, it is seen to consist of a colourless fluid filled with numerous little bodies, commonly called “blood-globules,” varying very greatly in size and shape, according to the animal from which they were taken. Those of the reptiles are very large, as may be seen at Fig. 4, Plate X., which represents a blood corpuscle of the Proteus. In this curious reptile the globules are so large that they may be distinguished during its life by means of a common pocket lens. ”

“ The Membrane of the Central Capsule.—The capsule-membrane or envelope of the central capsule is both morphologically and physiologically one of the most important parts of the Radiolarian body, for it separates its two main constituents, the capsule with its nucleus and endoplasm and the extracapsulum with the calymma and exoplasm. The capsule-membrane is invariably present at some time or other during the life of the organism, even though in a few species it may persist only for a short time. ”

“ This body is called the nucleus of the cell, and the small one within it, the nucleolus.
The membrane surrounding the cell is known as the cell wall, and in young plant cells is always composed of cellulose.
The granular substance lining the cell wall (Fig. 1, pr.) is called “protoplasm,” and with the nucleus constitutes the living part of the cell. If sufficiently magnified, the granules within the protoplasm will be seen to be in active streaming motion. This movement, which is very evident here, is not often so conspicuous, but still may often be detected without difficulty. ”

“ In the nuclei of Fig. 20 with the dark outlines, and of Fig. 7 a small reticular body is seen just opposite one end of the axis, or opposite both ends in g. In d (Fig. 20) this body is seen next the axis just below (outside) the hollow cup represented by the hollow ring. In this instance a central granule is seen in the reticular body, as also in c. I take this reticular body to be the centrosphere, and the central granule in c and d the centrosome. ”

“ So Nature shapes her hyaline vesicles and modifies them to serve the needs of the part where they are found. The artisan whirls his rod, and his glass bubble becomes a flattened disk, with its bull's-eye for a nucleus. These lips of ours are all glazed with microscopic tiles formed of flattened cells, each one of them with its nucleus still as plain and relatively as prominent, to the eye of the microscopist, as the bull's-eye in the old-fashioned windowpane. Everywhere we find cells, modified or unchanged. ”

“ To find a cell enclosing all that belongs to each individual, for example, every part of each lady’s dress, however complexly it may be folded among portions of the equal and similar dresses of neighbours; or, every twig, leaf, and rootlet of each one of the homogeneous assemblage of trees; we must alter the boundary by17 give-and-take across the plane faces of the primitive parallelepipedal cells, so that each cell shall enclose all that belongs to one molecule, and therefore (because of the homogeneousness of the partitioning) nothing belonging to any other molecule. ”

“ The spindle fibers have bulged out on each side of the old mother-cell nucleus, reaching the cell wall on each side. They have contracted away from the daughter nuclei, and seem at their outer ends to be in the process of gradual transformation into the normal reticulum of the cytoplasm. No visible thickening occurs on the fibers where the new plasma membrane is to be formed, but a space free of granules and stainable matter clearly shows where the new plasma membrane delimiting the two cells will form. This is particularly well shown in a photomicrograph of the cell shown in figure 63. ”

“ The form and structure of the nucleus vary greatly in different species. There are elongate, kidney-shaped, or even branched nuclei as well as spherical or oval ones. In addition to vesicular nuclei with a distinct karyosome and incidentally also with a nuclear membrane, homogeneous and more solid formations are frequently encountered. The nuclei are always differentiated from the protoplasm by their reactions, particularly in regard to certain stains. ”

“ Spherical body quite pellucid, like a glass globule, colourless. Central capsule with a moderately thick, but quite transparent, structureless membrane, its diameter ten to twelve times as large as that of the central spherical nucleus. Wall of the vesicular nucleus thick, perforated by fine pore-canals; on its inside numerous small spherical nucleoli. No large oil-globules in the movable protoplasmic network between the large alveoles. Extracapsular jelly-envelope very thin, containing no yellow bodies. ”

“ The fibres easily break across at the joints; and the last few segments are often narrowed and rounded, so as to form a blunt point. When they have been left in water, they exhibit colourless tubular sheaths surrounding and extending beyond them. These sheaths consist of the consolidated outer portions of the cell-walls; for when the cells undergo transverse division, and expand by growth in the direction of the length of the filament, the original septa or inner walls are broken through, and their remains may often be seen on the inner surface of the sheath, appearing as little teeth. ”

“ The bundle-sheath of the young stem is more easily recognised than in the older stem. It is, in fact, a continuous layer of cells, whose radial walls have a characteristic dark spot on each radial wall. The bundle-sheath lies immediately outside the vascular bundles, curving slightly towards the centre of the stem in the spaces between the bundles. It is more prominent in the stem when very young, as the cells are then filled with starch granules. This layer of cells will be readily seen in sections treated with iodine. ”

“ When these conditions are met, the black dots will appear in the emulsion directly above the cell or cell part from which the radiation came (see Figure 14) .
Shortness of range is satisfied by use of tritium, since its beta particles travel only about 1 micron (one thousandth of a millimeter) and the diameters of mammalian cells range from 15 to 40 or more microns. A mammalian-cell nucleus is at least 7 to 8 microns in diameter.
Figure 15 Cells being prepared for autoradiography. (a) Cells being coated with a photographic emulsion. (b) Coated cells being exposed to produce a latent image. ”

“ Reticular Tissue.—This is a variety of connective tissue in which the reticulum of white fibres is built up of very fine strands leaving large interspaces in which the cells typical of the tissue are enclosed. The ground substance of the tissue is reduced to a minimum. Many connective tissue cells lie on the fibres which may in places be completely covered by them. This tissue therefore forms a groundwork holding together the main parts of an organ to form a compact whole. ”

“ The membranogenous substance, the chloride of calcium, diffuses uniformly on all sides from the solid nucleus, and forms an osmotic membrane where it comes into contact with the solution. This spherical membrane is extended by osmotic pressure, and grows gradually larger. Since the area of the surface of a sphere increases as the square of its radius, when the cell has grown to twice its original diameter, each square centimetre of the membrane will receive by diffusion but a quarter as much of the membranogenous substance. ”

“ Inasmuch as this reticulum is perfectly continuous with the precisely similar though denser tissue in the ectoderm and endoderm, it may well be that a portion of the nerve trunks should be described as being ectodermal and endodermal in origin, though the bulk of them are undoubtedly formed from that portion of the reticulum commonly described as mesoblastic. But, however that may be, the tissue from which the great nerve trunks are developed is continuous on all sides with a similar tissue which pervades all the organs of the body, and in which the nuclei of these organs are contained. ”

“ The reticulum of channels of the plasmodium becomes meanwhile less and less marked. When the whole of the protoplasm is drawn in to the lobes, the circulation ceases. The lobes are the young sporangia. Meanwhile foreign bodies, taken in with the food, are ejected, and the protoplasm secretes on its outer surface a pellicle of mucoid, transparent substance which dries as the sporangia ripen. This invests the young sporangia, and as they rise above the substratum falls together at their bases forming the stalks ”

“ Materials and Methods The total number of circulating reticulocytes was calculated as the product of the concentration of circulating reticulocytes in the blood and the blood volume of mature animals for each species. ”

“ Examined microscopically, the parenchymatous envelope of the rootlets is seen to consist of two layers, the inner forming a small nucleus sheath. The outer portion is built up of large cells, loaded with starch granules and tufted crystals of oxalate of calcium. Salts of iron do not alter the tissue. ”

“ A nucleus as such is not present in bacterial cells, except in a few large rare forms and in very young cells. Nuclein, the characteristic chemical substance in nuclei, which when aggregated forms the nucleus, is scattered throughout the cell contents and thus intimately mingled with the protoplasm, and cannot be differentiated by staining as in most cells. ”

“ These artificial cells are sensible to most of the influences which affect living organisms. Like living cells they are influenced both in their organization and in their development by humidity, dryness, acidity, or alkalinity. They are also greatly affected by the addition of minute quantities of chemical substances either to the gelatinous blastema or to the drops which represent the primary nuclei. We may in this way obtain endless varieties, nuclei which are opaque or transparent, with or without a nucleolus, and cells containing homogeneous cytoplasm without a nucleus. ”

“ Prognosis. The prognosis is always fatal.
Lesions. When the foreign body is very thin and sharp, the reticulum may not become attached to the diaphragm. In such cases its passage has been rapid and the tissues have healed.
Usually the reticulum, diaphragm and pericardium are united by a mass of fibrous tissue as thick as a man’s arm. It resembles a fibrous sleeve surrounded by an œdematous zone, usually of slight extent. This mass of new fibrous tissue is traversed by a sinuous tract resulting from the irritant action of the foreign body on the surrounding tissues. ”

“ The surface of the body can scarcely be called rough, for, though it is reticulated, the skin is delicately smooth, glossy, and iridescent. The reticulations are produced by longitudinal and transverse lines, the former about 1/12th, the latter 1/8th of an inch apart, very regular. Both series are indented striæ, becoming evanescent by being pressed out, when the body is swollen or bent. The hinder extremity, for about an inch, is nearly smooth, forming a swollen oval sac, the furrows of both series being lost on its upper half in irregular corrugations. ”

“ All my specimens have been hardened with osmic acid, and with specimens hardened with this reagent it is quite easy to detect even the very smallest hole in a mass of cells.
As a solid string or rod of cells the Oviduct remains for some time; it grows, indeed, rapidly in length, the extreme hind end of the rod being very small and the front end continuing to remain attached to the knob. The knob, however, travels inwards and approaches nearer and nearer to the true pleuro-peritoneal [Pg 99] cavity, always remaining attached to the intermediate cell mass. ”

“ When the glands are exhausted by alcohol and potash, and broken by pressure between flat pieces of glass, they separate into individual cells which swell up slightly, while the membranous envelope is completely detached, and appears as a simple coherent film. After this treatment the cells, but not their membranous envelope, acquire by prolonged contact with strong sulphuric acid and iodine water a more or less brown or blue colour: the walls of the cells alone correspond therefore to cellulose. ”

“ The Desmidiaceæ consist mostly of single cells (Pl. V. figs. 9, 16) ; and these consist of two equal halves or segments, as indicated either by a paleness of the endochrome or a deep constriction at the line of junction, which is called the suture. The cells are often elegantly lobed and cut, or spiny; and in many the surface exhibits minute markings, consisting of little protrusions of the cell-wall outwards, or inflations, as they are called. ”

“ Retort for Chemical Experiments.—Plate 3, fig. 9, represents a combination of a large and a small tube, forming a retort, which can be employed with much advantage in many chemical experiments. When a gas is to be distilled by means of such a vessel, the ingredients are put into the wide tube, which is previously closed at one end, and then the other end of the 77 tube is either drawn out or soldered to a narrow tube. ”

“ It is wholly inconceivable that a homogeneous mass of all the varying chemical compounds which are contained in any given quantity of protoplasm could either exist or produce any regular sequence of chemical reactions. Structure, or organization of the cell-contents into separate colloidal compartments, and the segregation of cell-contents into masses having different functions, is essential to any reasonable conception of how the cell performs its various activities. ”